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KMID : 0351819980390020173
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Kyunpook University Medical Journal
1998 Volume.39 No. 2 p.173 ~ p.181
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Correlation of Lung Vascular ICAM-1 Expression and TNF-α after Exposure to Lipopolysaccharide
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Lee Chang-Ki
Woo Sang-Gyun
Park Jae-Hong
Kim Sang-Gi
Kim Byung-Suck
Jung Si-Wook
Choi Hee-Jin
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Abstract
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Lung injury following systemic lipopolysaccharide(L.PS) ad~rninistration is neutrophil-dependent. The infiltration of inflammatory cells requires tissue Tr~IF- a and increased expression of adhesion molecules. This experiment was designed to evaluate the relationship between TNF- a and intercellular adhesion molecule-1(ICAM-1) in vivo. Sprague-Dawley rats were given lipopolysaccharide(15mg/kg), intraperitoneally. The lungs obtained from 2hours to Sdays after LPS administration. Histopathologic and molecular biologic studies were done. LPS induced an early(2hours) increase in TNF- a and IL-1 a mRNA expression, followed by a peak at 6hours, with relatively stable expression at 2days. Histologically the inflammatory cellular infiltration and alveolar capillary congestion were noted from 2hour:s and progressively increased until 2days. Electron microscopically the alveolar capillary endothelial cells were swollen and fragmented. Using an immunohistochemical study, it was demonstrated that systemic LI´S administration caused a increase in ICAM-1 expression in F~ulmonary vascular endothelial cells. These data suggests that systemically exposed LI´S induces intrapulmonary up-regulation of ICAM-1 expression which is correlated with tissue TNF- a crverexpression.
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KEYWORD
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ICAM-1, TNF-a, LPS, plumonary inflammation
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